![]() The multimodal property of the ligands in the core ensures strong binding with most impurities over a wide range of pH and salt concentrations. The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of the various contaminants small enough to enter into the core. Capto Core 700 is built on the core bead technology which allows for dual functionality combining size separation with binding chromatography. Ensuring straightforward optimization and scale-up.Enabling significantly higher sample load.Relative to size-exclusion chromatography, Capto Core 700 provides the advantages of high purity, improved process economy, and increased productivity by: Capto Core 700 is a novel, layered bead chromatography resin consisting of a porous outer layer and ligand-activated core. After passing the Capto Core 700 column, the VLPs were disassembled into L1 protein monomers by disruption of disulfide bonds between the L1 proteins using dithiothreitol (DTT). Wide operational window of pH and conductivity. The pore size of the beads gives a restricted access to the ligand-activated core and provides a cut-off for molecules larger than Mr 700 000. The filtrate was run in flow-through mode on Capto Core 700 medium for removal of impurities. The core bead design of this medium gives dualfunctional properties that make it possible to combine size separation with binding chromatography. The Capto Core 700 beads consist of a ligand-activated core and inert shell. The core of each bead is functionalized with hydrophobic and positively charged ligands. The inactive shell aids in preventing large molecules (cut off Mr 700 000) from entering the core via the pores of the shell. The multimodal property of the ligand in the core ensures strong binding with most impurities over a wide range of pH and salt concentration. CaptoCore 700 comprises a ligand-activated core and inactive shell. The performance of the medium can be compared with gel filtration chromatography, but Capto Core 700 offers improved productivity and process economy. Find many great new & used options and get the best deals for GE HiScreen Capto Core 700 Intermediate Purification Polish Column 17548115 at the best online. Protein purity was calculated as the L1 protein-to-total protein ratio and expressed as a percentage.Capto Core 700 represents a new generation of chromatography media and is designed and optimized for purification of viruses and other large biomolecules. Capto Core 700 is a core-shell chromatographic support with an adsorbing core contained within an inert shell layer designed to purify larger biomolecules and bioparticles in a flow-through mode. Capto Core 700 is a multimodal chromatography medium that is designed for flowthrough purification of large proteins or viruses in vaccine processes.
0 Comments
Leave a Reply. |